Instruments
Malvern MicroCal PEAQ-iTC
Label-free in solution measurement of binding affinity and thermodynamics
MicroCal ITC isothermal titration calorimeters allow direct, label-free in solution measurement of binding affinity and thermodynamics in a single experiment, enabling the accurate determination of binding constants (KD), reaction stoichiometry (n), enthalpy (ΔH) and entropy (ΔS). This provides a complete thermodynamic profile of the molecular interaction. ITC goes beyond binding affinities and can elucidate the mechanisms underlying molecular interactions.
CHARACTERISTICS
- Affinity (KD)
- Enthalpy ∆H
- Entropy ∆S
- Stoichiometry (n)
- Sample volume: 280 µL
- Cell volume: 200 µL
- Injection syringe volume: 40 µL
- Injection volume precision: < 1% @ 2 µL
- Cell material: hastelloy
- Baseline noise: 0.15 ncal/s
- Temperature range: 2°C to 80°C
- Temperature stability: ± 0.00012°C
- 8-12 samples per 8 h day
- Up to 42 samples per 24 h (SIM) (automated version)
- Dimensions 430 x 380 x 460 mm (W, D, H) (calorimeter + wash station)
- Weight: 13.6 kg