INSTRUMENTS

Malvern MicroCal PEAQ-iTC

MicroCal ITC isothermal titration calorimeters allow direct, label-free in solution measurement of binding affinity and thermodynamics in a single experiment, enabling the accurate determination of binding constants (KD), reaction stoichiometry (n), enthalpy (ΔH) and entropy (ΔS). This provides a complete thermodynamic profile of the molecular interaction. ITC goes beyond binding affinities and can elucidate the mechanisms underlying molecular interactions.

CHARACTERISTICS
  • Affinity (KD)
  • Enthalpy ∆H
  • Entropy ∆S
  • Stoichiometry (n)
  • Sample volume: 280 µL
  • Cell volume: 200 µL
  • Injection syringe volume: 40 µL
  • Injection volume precision: < 1% @ 2 µL
  • Cell material: hastelloy
  • Baseline noise: 0.15 ncal/s
  • Temperature range: 2°C to 80°C
  • Temperature stability: ± 0.00012°C
  • 8-12 samples per 8 h day
  • Up to 42 samples per 24 h (SIM) (automated version)
  • Dimensions 430 x 380 x 460 mm (W, D, H) (calorimeter + wash station)
  • Weight: 13.6 kg