Biosensing Instrument BI-4500 Series

High throughput with 5 channels flow modes SPR

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The 5-channel Surface Plasmon Resonance (SPR) instrument, combined with the BI Autosampler, is the ideal solution for high throughput assay development. With the BI-DirectFlow™ technology, it provides precise sampler delivery and high-quality binding kinetic measurements.


High throughput with 5 channels and fully automated Autosampler Provides 5 channel flow modes and high sample capacity with up to two 384 well plates.

High sensitivity Delivers high quality binding response for low immobilization and small molecule (< 100 Daltons) detection.

Precise sample delivery with BI-DirectFlow™ Technology Delivers sample with near-zero dispersion for fast kinetics and effective removal of various secondary effects.

Temperature controlled SPR detection module Covers wide SPR angle range for measuring SPR shift in liquid phase. Precision background and reference subtraction. Temperature control for thermodynamic studies, 6 °C to 50 °C.

Innovative multi-module design Provides users with maximum flexibility to choose amongst various analysis modules for electrochemistry and liquid applications.

Characteristics / System Specifications

Base Station
Light source 670 nm dual light sources
Detection speed 4 ms ( > 200 data points per second)
Incident angles 40-47 Deg (gas)
67-81 Deg (liquid)
Baseline noise ~ 0.04 RU RMS (< 0.01 mDeg RMS)
Baseline drift 0.30 RU/hour (0.05 mDeg/hour)(when ambient drifts < 1°C/hr)
Temperature Control Range 6°C to 50°C (10°C below ambient temperature max)
Outer dimension 355(w) x 250 (h) x 515 (d) mm
Weight 11.5 kg
Power supply 110-230 V 50/60 Hz

Fluid Handling

Number of sample flow channels 5 channels
Flow cell material PEEK (biologically compatible)
Flow rate 1.0 to 250 μL/min (application dependent)
Sample injection volume >50 μL (application dependent)
Sample injection methods Fully automated (with Autosampler option)
Kinetic constant k a < 1 X 108 M-1s-1; k d > 1 X 10-6 s-1
Dissociation constant KD = 10-3 M (1 mM) to 10-12 M (1 pM)
Molecular weight cutoff 100 Da
Analysis Modules 5-channel BI-DirectFlow™ module (installed)
Option: EC SPR module and Gas SPR module

Control System
Computer Windows operating system
Software includes: Real-time contral SPR software; Data Analysis software; Kinetics Analysis software

Sample capacity
Up to 768 samples with various vial tray size including 2 x SBS standards (384 / 96), 2 x 48 Vials (1.5mL), 2 x 12 Vials (10mL)
Injection volume 20uL to sample loop volume (1500uL)
Sample cooling Minimum: 4oC +/- 2oC
Outer dimension 300 (w) x 575 (h) x 360 (d) mm
Weight 21 kg

PP-100 Auto buffer exchange pump
Buffer exchange Automatic buffer exchange up to 6 buffer sources
Buffer degasser In-line
Buffer delivery Continuous
Outer dimension 305 (w) x 191 (h) x 330 (d) mm
Weight 6.8 kg