Biosensing Instrument SPRm 200 Series
Powerful Surface Plasmon Resonance Microscopy (SPRM) system tailored to your unique research needs
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The Surface Plasmon Resonance Microscopy (SPRM) system, integrates optical microscopy and SPR, is a powerful technique for measuring binding activities of membrane proteins in vitro. It allows the simultaneous measurement of phenotypical changes of the sample via bright field and binding strength and kinetics via SPR.
Features
Integrated optical microscopy with SPR Bright-field and SPR microscopies in one instrument. Large field of view with high resolution optics to view single or multiple cells.
In vitro and label-free binding activities mapping Provides SPR sensorgrams and binding activity map of individual cells as well as the binding kinetic constants (ka, kd, KD).
Nanometer scaled binding response of virus, bacteria and nanoparticles Nanomotion detection study of bacteria or virus metabolic activity or binding activities of nanoparticles for drug delivery designs.
Analysis Modules include BI-DirectFlow™ Module for advanced five-channel ultra-fast kinetics research
Characteristics / System Specifications
Base Station
Light source 690 nm
Incident angles 40-76 Deg (continuous)
Baseline noise < 0.6 RU RMS (0.1 mDeg RMS)
Baseline drift 3 RU/hr (0.5 mDeg/hr) (when ambient drifts < 1°C/hr)
Temperature Control Range 15°C to 40°C (10°C below ambient temperature max)
Field of view Bright Field: 1200 x 900 um
SPR: 600 x 450 um
Magnification Bright Field: x10
SPR: x20
Resolution Bright Field & SPR: 1 μm
Stage translation / rotation 3mm x 3mm / 360 deg
Outer dimension 690 (w) x 330 (h) x 340 (d) mm
Weight 23 kg
Power supply 110-230 V 50/60 Hz
Fluid Handling
Sample volume 1 to 1500 μL (application dependent)
Kinetic constant ka <1 X 107 M-1s-1
kd >1 X 10-5 s-1
Dissociation constant KD = 10-3 M (1 mM) to 10-12 M (1 pM)
Molecular weight cutoff 200 Da
Control System
Computer Windows operating system
Software ImageSPRTM software including Data Analysis and Kinetics Analysis
Autosampler (option)
Sample capacity 2 x SBS standards (384 / 96), 2 x 48 Vials (1.5mL), 2 x 12 Vials (10mL)
Sample cooling Minimum: 4C +/- 2C
Outer dimension 300 (w) x 575 (h) x 360 (d) mm
Weight 21 kg
Automatic Buffer exchange pump and degasser (option)
Buffer exchange Automatic buffer exchange up to six sources
Buffer degasser In-line
Buffer delivery Continuous
Outer dimension 305 (w) x 191 (h) x 330 (d) mm
Weight 6.8 kg
Optional Accessories
EC-DualFlow™ Module for advanced two-channel electrochemical (EC) flow SPR research
EC-SPR Module for electrochemical (EC) SPR research
Gas SPR Module for gas phase and chemical vapor SPR research
Autosampler Automate your BI SPR experience compatible with 384, 96, 48, and 12 plate formats
BI Sensor Chips with high uniformity for reproducible SPR research
